A.I.G. will have to unwind derivatives contracts, a process that involves paying big termination fees.
But again, this would create a big "termination risk" only if the rest of the world allowed the geoengineering to go on for decades.
She noticed, when she worked on big termination packages after mergers, that men would feel their worlds were collapsing while women's egos were "more diversely invested".
The ESTs were sequenced at the University of Georgia sequence center from the 3' polyA tails (Athens, GA) with Big Dye termination protocols using ABI 3700 instrumentation (Applied Biosystems, Foster City, CA) resulting in about 4500 reads.
Sanger sequencing was performed using the Big Dye termination kit.
Briefly, Big Dye Termination Chemistry and a series of 72 primers were used to sequence in both forward and reverse directions.
The purified products were then sequenced by using the Big Dye Termination Cycle Sequencing Ready Reaction Kit 3.1 (Applied Biosystems, Foster City, CA, USA), according to the manufacturer's protocol, with subsequent analysis on an Applied Biosystems 377 DNA automated sequencer.
Direct nucleotide sequencing of N (261 bp) and Gn (324 bp) PCR products was performed by using the Big Dye Termination Cycle Sequencing Ready Reaction Kit (Applied Biosystems, Foster City, CA, USA) with 25 cycles at 95°C, 55°C for 45 s, and 72°C for 180 s.
Complementary DNA was produced by a reverse transcription polymerase chain reaction with primers 10 g and 304 (19 ) and was sequenced by using the Taq Big Dye Termination Cycle Sequencing Ready Reaction Kit (Applied Biosystems, Foster City, CA), according to the manufacturer's protocol, on an Applied Biosystems 377 DNA automated sequencer (Applied Biosystems).
Purified PCR products were sequenced using the Big-Dye Termination cycle sequencing reactions and an ABI Prism 3100xl sequencer (Applied Biosystems, Inc., Foster city, CA).
The amplified regions were submitted to direct sequencing by the chain-termination method [ 45] using a Big Dye Terminator kit (v.3.1), which uses the enzyme AmpliTaq DNA (Life Technologies, CA, USA).
