Exact(1)
The cell suspension was mixed with 5 μg freshly prepared (Big BAC DNA kit, Princeton Separations) and nucleofected under protocol B-16 (Amaxa).
Similar(59)
"The post-bac is a big risk to take," says Marissa Lipton, who at 26 is in her second year of med school at the University of Connecticut, where she also completed her post-bac.
Choose the BAC with the biggest DNA array.
Devilishly good... Punchdrunk theatre, whose Faust was such a big hit, are to return to BAC.
Disappointing earnings reported by big banks like Citigroup (ticker: C) and Bank of America (BAC) also rankled, as did the failure of economic bellwethers like 3M (MMM), Honeywell (HON) and Caterpillar (CAT) to deliver earnings that met increasingly high hopes.
T7/Sp6 BAC ends were sequenced using the Big Dye terminator 3.1 system as described by BAC/PAC Resources [BACPAC Resources Center [ http://bacpac.chori.org/].
T7/Sp6 BAC ends were sequenced using the Big Dye terminator 3.1 system as described by BAC/PAC Resources [ 33].
· Coming up: BAC Octoberfest, BAC Opera 2003, an adult Christmas musical.
They are betting partly on union divisions to undermine the strike: Laurent Berger, leader of the CFDT, a big union which backs the reform, has accused those on strike of "contempt" for bac candidates.
To analyse end sequences, BAC DNAs were amplified using a TempliPhi large construction kit (GE Healthcare, UK), and the end sequences were analysed according to the Sanger method using a cycle sequencing kit (Big Dye-terminator kit, Applied Biosystems, USA) with DNA sequencer type 3730xl (Applied Biosystems).
End sequencing of 19,957 BAC clones was performed using 2.5 μL of BAC DNA and 1.0 of Big Dye 3.1 Terminator Ready Reaction Mix (Applied Biosystems, Foster City, CA), for a total reaction volume of 10 μL.
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