Sentence examples for bidirectional sequencing of the from inspiring English sources

The genetic study was performed by PCR amplification followed by direct bidirectional sequencing of the entire coding regions and intronic flanking sequences of the CACNA1A, ATP1A2, SCN1A, PRRT2 and NOTCH3 genes.

Standard bidirectional sequencing of the inserts confirmed the incorporation of various point mutations.

The specificity of amplification was verified by bidirectional sequencing of the product.

Bidirectional sequencing of the PCR products was conducted with each primer pair.

Bidirectional sequencing of the purified PCR products was performed on an Applied (3130XT) automated capillary sequencer (Applied Biosystems, Foster City, CA, USA) (protocols available on request).

Mutational analysis of EYA1 gene (NM_000503.4; GeneID: 2138) was performed by polymerase chain reaction (PCR) and bidirectional sequencing of the coding exons and intron/exon flanking regions.

In this cohort, eight DNA samples had the SNP by TaqMan genotyping, but only one was confirmed with additional bidirectional sequencing of these specific DNA samples.

Bidirectional sequencing of all the coding exons of BRAF in 15 well-characterized and highly utilized ovarian cancer cell lines identified four cell lines with BRAF mutations.

This facilitates bidirectional sequencing of both the sense and antisense template strands during one paired-end read, which maximizes target coverage.

We performed PCR reactions using the Qiagen Multiplex PCR kit, and bidirectional sequencing of resulting amplicons using the BigDye terminator cycle sequencing kit and an ABI3730 automated sequencer (ABI PerkinElmer).

Bidirectional sequencing of all coding exons and splice sites of the human genes MYH6 and MYH7 was carried out for the index patient III:4 of family DU-11.