Bidirectional sequencing was performed using MegaBACE 1000 sequencer (GE Healthcare).
Bidirectional sequencing was performed by an ABI automated cycle sequencer using an ABI BigDye Terminator Cycle Sequencing Kit (Applied Biosystems, USA).
The sequencing error rate was 2- to 3-fold higher in/around homopolymeric tracts, and could be discerned from true polymorphism using bidirectional sequencing.
In this study, we analyzed the promoter region of the GATA4 gene by bidirectional sequencing in 172 VSD patients and 171 healthy controls.
Stage 2 Canadian and Dutch cases were also genotyped using bidirectional sequencing.
The sequence was confirmed by bidirectional sequencing and comparison to the mouse genome.
Emulsion PCR amplification was carried out using either primer A or primer B or both for bidirectional sequencing.
The assay was validated by bidirectional sequencing in 50 subjects, and both methods gave 100% identical results.
The mRNA was then used for detection of the JAK2 Δexon14 variant by RT-PCR with bidirectional sequencing.
We carried out a mutation screening of HOMEZ with bidirectional sequencing in a subset of Indian probands (n = 54).
The smooth muscle MD GFP chimera vector was confirmed by bidirectional sequencing and produced a 116 kDa protein when expressed in the coupled translation assay.
