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The dual-channel bidirectional design allows for two channels each running at 10 gigabits per second.
The semisymmetric bidirectional design was used in only one study.
Overlap bias is avoided by the semisymmetric bidirectional design [ 20].
For case-crossover analysis, Navidi modified his bidirectional design (Navidi 1998) and proposed the semisymmetric bidirectional design (Navidi and Weinhandl 2002).
The symmetric bidirectional design with selection of two controls, seven days before and after the index day (day of dispensing glyceryl trinitrate) was used [ 20].
In summary, our results demonstrated the advantageous properties of the autoregulatory compared to the self-contained principle for M2-transactivator expression, when using retroviral vectors with a bidirectional design, combined with the inducible cA-promoter.
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The most frequently used bidirectional designs were symmetric (42.2% of studies) and time stratified (48.9% of studies).
We observed an ongoing effort to perfect the CCO design dating from the initial unidirectional design up to the bidirectional designs with their subtypes.
Our review of methodological aspects revealed a trend in CCO bidirectional designs with regard to the choice of control periods (Table 1).
A systematic review on case-crossover analysis showed that only 7.7% used unidirectional design and the remainder bidirectional, most commonly using symmetric or time-stratified bidirectional designs (91.1% combined) [ 51].
Here we applied bidirectional promoter design in combination with TAS in vitro and in vivo.
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