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Using only chemical synapses, we tested our three major predictions from our model concerning degree, connection lengths and bidirectional connectivity.
As expected for parallel growth, group 1 versus group 2 did not display significantly higher degree, long-range connection lengths or bidirectional connectivity differences.
When curves followed close to a power law (connection probability and bidirectional connectivity), double-logarithmic axes were used and fitted with linear models.
Topological and spatial properties between serial and parallel growth such as out-degree, local efficiency, connection probability and bidirectional connectivity were averaged over 50 trials for each neuron then fitted with exponential or polynomial curves.
For smaller neuron sizes such as radii 0.5 and 0.604 concomitant with 1 and 2 maximum incoming connections allowed, respectively, rewired networks did not have any bidirectional connectivity even when considering larger numbers of neurons up to 1,800, or higher neuronal density.
In networks characterised by bidirectional connectivity, this is known as diffusive coupling.
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We report that pyramidal neurons form a sparsely connected (6.7% connectivity) network with an overrepresentation of bidirectional connections.
To compare connectivity rates between in vivo and in vitro and to test whether bidirectional connections are overrepresented (i.e., non-random connectivity), we performed two-tailed chi-square tests.
Our SEM results provide valuable support for this account: the best-fit models of conjunction-task connectivity (Fig. 7A C) required a bidirectional connection between DLPFC and VLPFC regions that were found to be more active during conjunction- than single-task trials.
In addition to these methods, Senel et al. [26] used MDCs to restore network connectivity, but the MDCs are only moved back and forth between different partitions to establish bidirectional connection and do not bring any directly acquired data back to the sink node.
Bidirectional connections were more numerous for parallel growth than for serial growth, which we confirmed with C. elegans connectivity data; the discrepancy between neurons (or neuron groups) was negatively correlated to the degree of overlap in the developmental time windows.
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