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In contrast, no marked difference was observed in the levels of Bid expression between GFP- and Cre-expressing erk5loxP SCG neurons up until 72 h post-infection.
Furthermore, changes in Bcl-2, Bax, and Bid expression were detected.
We reduced Bid expression in Bim−/−/Puma−/− MEFs using shRNA (Figure 5B).
Our findings therefore support the data from a previous study of Borner and co-workers [50] where Bid expression was not required for ER stress mediated cell death.
In the present study, we find that cells collectively deficient in Bim and Puma with reduced Bid expression still die in response to NO.
Given these special characteristics of Bid compared to other BH3-only proteins, we established a stable knockdown of Bid expression by a gene silencing approach in HeLa cervical carcinoma cells and addressed the functional importance of Bid expression in death receptor- and stress-induced apoptosis in a human cancer cell line.
We first detected Bid expression and activation.
No change in Bid expression level was observed.
Bid expression was analysed by western blot and immunofluorescence assays.
BID expression was reduced in cell lacking BNIP3.
Again, there was no effect of Bid expression on proliferation.
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