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The Bid backbone had a thrombin cleavage site in place of the caspase 8 cleavage site to allow one-step cleavage and purification and was cloned into pGEX-4T2 to add a C-terminal HA tag.
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Superimposition of bound Bak and Bid BH3 backbones indicate that both ligands are bound in near identical manner (Supplementary Figures 3a and b).
"We're excited to be joining forces with the talented staff who provide the current service in the Tyne Tees and Border region and in Wales to create the backbone of our bids for the pilots announced today," said John Hardie, the ITN chief executive.
These other interactions, likely critical for substrate recognition, were hydrogen bonds between: Bid D55 and JNK2 residues R107/K191/N194, and Mg2+; Bid L57 (backbone) and V225/T226; Bid Q58 and K191/S193/T226; and Bid G61 (backbone) and R63 to C154.
Our report is now being used as the backbone of decision making at the highest level, as these potential locations work to develop their regional bids for a spaceport.
A backbone".
They are the backbone.
He wants backbone.
He has no backbone".
"Some people have backbone.
They have backbone.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com