Sentence examples for bicarbonate solution was added from inspiring English sources

Fifty microliters of 1 M sodium bicarbonate solution was added into a 0.5 mL of 100 μM BSA and LYZ solutions.

Carbon dioxide gas or sodium bicarbonate solution was added to the circuit to maintain the pH of the circulating blood in the range 7.25 7.55.

Ten ml of sodium bicarbonate solution was added to this and ZD2767P hydroiodide salt was converted to ZD2767P di-sodium salt.

Then, 750 μl of 60 g/l sodium bicarbonate solution was added and further incubated in the dark for 90 min at 30°C.

After 5 min, 1.0 ml of 7.5 % sodium bicarbonate solution was added to the mixture and allowed to stand for 90 min at room temperature in the dark.

An ammonium bicarbonate solution was added to the samples to a final concentration of 100 mM supplemented with 1 mM calcium chloride.

Then 0.75 mL of sodium bicarbonate (60 mg/mL) solution was added and mixed thoroughly.

Then, 400 μl sodium bicarbonate (60 mg/ml) solution was added and the mixture was allowed to stand at room temperature for 90 minutes.

After virus adsorption, the inoculum was removed and carboxymethylcellulose (CMC) overlay medium consisting of cell culture medium supplemented with 5% heat inactivated FBS, 3% sodium bicarbonate (Invitrogen), 1% antibiotic/antimycotic solution was added.

The vaccine was diluted at a concentration equivalent to 3 µg/ml total HA in 0.1M sodium bicarbonate buffer and 50µl of this solution was added to each well of the ELISA plates and incubated overnight at 4°C.

To cover the gel piece, 5 μL of 40 mM ammonium bicarbonate in a 9% acetonitrile solution was added and incubated overnight at 37°C.