Sodium bicarbonate (NaHCO3) method was used for available P determination (Olsen et al. 1954) while total P was determined through vanadomolybdophosphoric yellow color method (Jackson 1973).
Both Melbourne and Western Health units phased out an oral bicarbonate supplementation method.
We studied flight metabolism using the C-labelled Na-bicarbonate (NaB) method, modified for bolus injections in flying endotherms [ 6].
In these studies, carried out on a small number of patients with a pathologically decreased but stable serum level of bicarbonates, their method of calculation indicated a positive balance of protons.
Ions such Na, K, Ca, Mg, Cl and SO4 − were analyzed using ion chromatography (ICS1100) and bicarbonate using volumetric titration method (Sulfuric acid method).
Serum electrolytes concentration was determined by established methods; sodium and potassium concentration by flame photometry, chloride and bicarbonate concentration by titrimetric method, and calcium concentration by cresol phthalein complexone method [ 22- 24].
Additionally, there is potential utility for systemic bicarbonate as a safe method for alkalinizing highly protonated chemotherapeutics against acidic tumors.
In vitro cultures of the W2 strain of P. falciparum were grown in group A+ RBCs at 4% hematocrit in Royal Park Memorial Institute (RPMI) 1640 medium supplemented with 4- 2-hydroxyethyl -1-piperazineethanesulfonic acid (HEPES), human plasma, and sodium bicarbonate following the described method [ 7, 8, 14- 2-hydroxyethyl -1-piperazineethanesulfonic
Fig. 1A shows a photoresponse family recorded by the ERG technique across an isolated dark-adapted mouse retina perfused with bicarbonate-buffered (see Methods) Ringer solution at 37°C, when the ON-bipolar cell activity was suppressed by 50 µM DL-AP4.
Calcium, magnesium, chloride, carbonate, and bicarbonate were determined by titrimetric method.
