Sentence examples for bicarbonate buffer was added from inspiring English sources
The dose of strain TW10598 was suspended in 2 ml PBS, to which 30 ml bicarbonate buffer was added just before drinking.
Then, 50 mM ammonium bicarbonate buffer was added again (pH 7.8), and the mixture was incubated at 37°C for 12 14 hours.
A solution of 1 M ammonium bicarbonate buffer was added to PNGase F to bring the final pH to 8, close to the optimal pH for deglycosylation by PNGase F. A solution of 1 mM DP7 was spiked into the enzyme solution as an internal standard to obtain a final concentration of 70 mM.
Each sample containing an individual band was rehydrated in 10 15 μL of solution containing 10 ng/μL of trypsin or chymotrypsin (Promega) in 50 mM ammonium bicarbonate for 15 min. Fifty microliters of 50 mM ammonium bicarbonate buffer was added to each sample, and the samples were incubated at 37 °C for 18 h.
Briefly, 250 μL of FITC (1 : 20 diluted in 0.1 M carbonate-bicarbonate buffer) was added dropwise to 1 mL antibody solution (5.0 mg/mL in 0.1 M carbonate-bicarbonate buffer, pH 9.0) while stirring the reaction vial, followed by incubation at room temperature for 2 hrs with gentle stirring.
Volumes were adjusted to 100 µL with carbonate-bicarbonate buffer and 150 μL of a reaction buffer was added into each well.
Then, ATP assay buffer was added.
Formamide loading buffer was added.
Using a peristatic pump, ten milliliters of Au NPs (3.4 × 1012 particles/mL) in sodium bicarbonate buffer were steadily added at 7 μL/s to 2 μL of 20 mM SPDP solution in dimethyl sulfoxide (DMSO) (4.73 × 10−20 mol SPDP per Au NP) while the solution was stirred vigorously on ice.
A bicarbonate buffer system was added to the integrated model in the blood compartment to balance protons.
