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All diets were balanced for protein and minerals and supplemented with monensin (4 ppm) and sodium bicarbonate (1%).
The first one demonstrated that hydration with 0.9 % saline was associated with a lower decrease in GFR and a lower incidence of CIN as compared with sodium bicarbonate (1 vs 9 %, p = 0.02) [134].
These clay particles were dispersed in water and heated at 75°C in the presence of a solution composed of the sodium salts of bicarbonate (1 M), citrate (0.3 M), and chloride (2 M) (Pusino et al. 1989; Robert and Tessier 1974).
The protein solution was concentrated to ∼10 µm followed by dialysis into 10 mM ammonium bicarbonate, 1 mM magnesium acetate, pH 7.8.
The liver was perfused with Krebs Ringer Buffer (KRB; 7.13 g/L sodium chloride, 2.1 g/L sodium bicarbonate, 1 g/L glucose, 4.76 g/L HEPES and 0.42 g/L potassium chloride) that contained 1mM EDTA (ethylene diamine tetra acetic acid), followed by perfusion with a 0.1% w/v collagenase (Sigma, Type IV).
Madin-Darby canine kidney (MDCK; ATCC, CRL 1708) and Vero E6 cells were cultured in Eagle's minimal essential medium supplemented with non-essential amino acids, 100 IU/ml penicillin, 100 µg/ml streptomycin, 2 mM L-glutamine, 2% sodium bicarbonate, 1% HEPES, and 10% Fetal Bovine Serum (FBS) (all from BioWhittaker, Verviers, Belgium).
Similar(21)
Calcium carbonate deposits dissolve in water that contains carbon dioxide to form calcium bicarbonate, Ca HCO3 2.
Sodium bicarbonate 2.8 g was given.
The guidelines do not recommend administration of sodium bicarbonate [12].
Bicarbonate (HCO3) was estimated by titration with H2SO4 standard solution.
Bicarbonate (HCO3−) are determined by titration with HCl.
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