Exact(7)
The availability of two independent ATH1 microarray datasets investigating natural genetic variation in transcript accumulation within Arabidopsis allows a replicated analysis of metabolic pathways to detect biased transcript variance.
Moreover, given ejac-sp is a male accessory gland biased transcript, primers specific to this transcript would not amplify products in the other tissues, thus eliminating our ability to test if ejacsp-dsRNA's effect was specific to the trypsin-like serine proteases in the male accessory gland or all tissues.
The homoeologues of TaSTOP1 exhibited similar genomic structures, but showed biased transcript expression and different response to Al and proton (H+) toxicity.
The three homoeologues of TaSTOP1 have similar genomic structures, but showed biased transcript expression and different response to Al and proton (H+) toxicity.
We have demonstrated that alignment of RNA-seq reads to a reference genome can result in biased transcript abundance estimates, much in the same way that a reference-based probe design can bias hybridization of genetically diverse samples to microarrays (Walter et al. 2007).
We find that the most common structural differences among the random pairs of female- and male biased transcript isoforms are those at the 5' end of a gene.
Similar(53)
In order to understand the regulation of sexually dimorphic genes in human and mouse kidneys, we examined transcription factor binding sites (TFBS) on the sex biased transcripts.
Supplemental Table S7 gives the details for each of these sex biased transcripts ranked by fold-difference.
Gene ontology analysis for the 3304 female biased transcripts compared to all expressed transcripts revealed many over represented classes (including regulation of metabolism, transcription, and zinc binding proteins) a few under represented classes (including the extracellular space).
The majority of the parentally biased transcripts we detected overexpressed the maternal allele.
Although our dataset did not examine reproductive workers, we did find some interesting maternally biased transcripts.
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