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Low DMP in C. glomerata may be explained by post-copulatory processes, such as biased fertilization of eggs with compatible sperm (in terms of sex alleles) [ 62].
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If this is the case then we would expect polyandrous females to possess mechanisms to bias fertilization toward compatible males (Bretman et al. 2004).
Recent work (Burdfield-Steel ER, unpublished data) suggests that these CHCs may play a role in mate choice and species discrimination in Lygaeus; hence, removal may limit the female's ability to bias fertilization toward conspecifics.
Here, demonstrations of epistatic fitness variation in conjunction with male × female interactions in female choice are insufficient: females must also be able to consistently bias fertilization success among males towards males that carry genes that are compatible with her own genes.
Finally, in our final experiment we found no evidence that CHCs played an important role in identifying compatible individuals or in facilitating the biasing of fertilization.
The biasing of fertilization can also occur during intraspecific multiple matings, for example, in copulations between different races of alpine grasshopper, in which a higher proportion of offspring from virgin females were sired by males from the same race as the focal female (Podisma pedestris: Hewitt et al. 1989).
Fertilization success values render biased sperm competitive ability values.
In such cases, fertilizations, under sperm competition, were heavily biased against the TF males (mean paternity of TF males = 27.3 ± 0.02%), indicating that the sperm produced by TF males was clearly outcompeted in competition with sperm of the tester males.
The realized outcrossing rate obtained here will be biased by any inbreeding depression resulting in seed abortion after fertilization as well as that operating on young seedlings arising from initially viable seed.
Experiments conducted in our laboratory have shown that even when the population sex ratio is strongly biased toward females in normal healthy populations (14 females:2 males), fertilization rates are > 90%.
Here I have shown that the relative nature of the calculation of fertilization success means that the inference of sperm competitive ability is biased.
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