It should be noted that incomplete compensation leading to homogametic-biased expression is not unique to the ZW system.
However, whether a relocated gene has male-biased expression is not independent of whether it is the result of →A or →X relocation (P < 0.05, F.E.T).
For instance, in our analyses, the transcripts of tango13 apparently display sex-specific splicing but their computed sex-biased expression is not deemed statistically significant.
In particular, Metta and Schlötterer used a dataset combined with expression data in which significant sex-biased expression is not detectable.
Because the degree of sex-biased expression is not strongly correlated between lineages, the male-biased genes that were examined were not those with very high levels of male-biased expression D. ananassae (Grath et al. 2009).
Our first observation was that all nuclearly encoded duplicates having biased expression are testis-biased (i.e., we did not find any gene having biased expression for any other tissue; supplementary table 3, Supplementary Material online).
However, the precise molecular mechanism underlying the allelic bias in gene expression is not very well understood.
Thus we conclude that the variation in miRNA expression is not biased by the array platform we used.
While this difference did not reach statistical significance (P = 0.083), the results may have been negatively biased as EGFRvIII expression was not a criterion for inclusion.
