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Previous reports have suggested that samples of <1000 cells can only generate reliable expression data for the most abundantly expressed genes, thereby leading to a <span class="lh">biased dataset [40].
Those codons that occur significantly more often (p < 0.01) in the highly biased dataset relative to the lower biased dataset are putatively considered optimal, and are indicated with a.
Subsequently, we randomly sampled SNPs from the biased dataset until a total of 1000 candidate SNPs were obtained.
We created a biased dataset by introducing a SNP every 100 bp into all genes and again randomly sampled 1000 SNPs.
On the final conclusion of the authors that the common view of influenza virus evolution as a positive selection-driven process is incomplete I am not convinced that the view of long periods of stasis punctuated by short periods of fitness described here is not primarily due to the use of a biased dataset.
Using a keyword based article selection has the risk of generating a rather narrow or biased dataset, especially when the aim is named entity recognition.
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McClain [4] suggested that hard substrate seamounts have received considerably more attention than soft bottom ones, which may result in biased datasets.
Integrated analysis was carried out to identify and exclude biased datasets [ 42, 43].
This is likely to be a result of our sampling scheme for creating biased datasets, which allows individuals to be present in a dataset more than once.
This strategy could complement the extraction of SSU-rDNA genes from PCR-free shotgun metagenomic datasets, and, within these less taxonomically biased datasets, ease the selection of SSU-rDNA genes with sequence similarities to (groups of) divergent V4.
Furthermore, such methodologies have been subject to errors incumbent within the nature of the protocol i.e. those protocols reliant upon DNA amplification generate biased datasets from which it is difficult to make firm conclusions [ 19].
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com