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The most striking and most conserved feature of the EDCRP amino acid sequence is the highly biased abundance of individual amino acids.
In this model, parasitism, commensalism and amensalism all lead to the exclusive survival of the species with higher fitness in a community, qualitatively consistent with the findings from the computational framework that the structures of the communities with asymmetrical interactions have a biased abundance towards the species with a growth advantage.
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Quantitative null models (e.g. Patefield's algorithm) control for sampling bias, abundance and diversity, and are thus important for the unbiased interpretation of changes in network metrics.
To test if the tendency toward multiacetylated proteins in the mitochondria is biased by protein abundance, we estimated the relative protein abundance for all proteins using the iBAQ method.
Current mass spectrometry techniques are unable to resolve all proteins in a complex mixture, such as that arising from higher eukaryotic cells, and are biased towards high abundance peptides [48].
Because the CLASH technique depends on the recovery and sequencing of crosslinked RNA, the results will be biased by transcript abundance.
For example, the relative position of an exon or intron within the gene annotation unit and the length of an exon or intron were not correlated with female- or male-biased abundance.
This could be due to two factors: firstly the low proportion of sequences incorporated into the contigs, (less than a third of all sequences were used for the HD contigs) and secondly the assemblies may be biased towards high-abundance transcripts (see Figure 3 – top right).
Thus, any volume determination carried out with this cell population will be inaccurate since it is strongly biased by the relative abundance of cells in G2, prophase and cytokinesis.
Thus, the detection of proteins is typically limited and biased towards the higher abundance molecules.
10 However, the use of peripheral blood (PB) mononuclear cells for transcriptional analysis may result in data that are biased by relative subset abundance.
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