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More recently, Raiford et al. [6] compared Wagner's [5] amino acid biosynthetic costs with codon bias, transcript levels and protein levels and observed that cost minimisation is observed across different functional categories of genes, but that its effects are restricted to certain amino acids.
These allelic variations between the two genotypes can bias transcript profiling by causing both false positives and false negatives [ 35, 36].
However, previous research involving cross-species hybridization (CSH) has shown that sequence variation between two species can bias transcript profiling by decreasing sensitivity (number of expressed genes detected) and the accuracy of measuring fold-differences in gene expression.
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Likewise, all seven male bias transcripts with >10-fold effects were not expressed in females and mapped to the Y chromosome.
The availability of two independent ATH1 microarray datasets investigating natural genetic variation in transcript accumulation within Arabidopsis allows a replicated analysis of metabolic pathways to detect biased transcript variance.
Moreover, given ejac-sp is a male accessory gland biased transcript, primers specific to this transcript would not amplify products in the other tissues, thus eliminating our ability to test if ejacsp-dsRNA's effect was specific to the trypsin-like serine proteases in the male accessory gland or all tissues.
The homoeologues of TaSTOP1 exhibited similar genomic structures, but showed biased transcript expression and different response to Al and proton (H+) toxicity.
The three homoeologues of TaSTOP1 have similar genomic structures, but showed biased transcript expression and different response to Al and proton (H+) toxicity.
In order to understand the regulation of sexually dimorphic genes in human and mouse kidneys, we examined transcription factor binding sites (TFBS) on the sex biased transcripts.
Supplemental Table S7 gives the details for each of these sex biased transcripts ranked by fold-difference.
Supplemental Table S8 gives a full listing and P-values for the gene ontology findings of the female biased transcripts.
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