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In this setting, our method produces a population of amplified RNA having minimal and consistent amplification bias, orientation compatible with both spotted cDNA and oligonucleotide arrays, and quantity sufficient for several downstream assays from a single amplification.
Taken together, the purine constraints are such that R1 > R2 and R1 > R3, which makes the purine bias orientation change from the first to third codon positions (Fig. 7) regardless of the secondary structure under consideration.
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Other acute remodeling evidence included smooth muscle cell hypertrophy and changes in smooth muscle bundle orientation from the longitudinally biased orientation in the normal bladders to the biphasic (both longitudinal and circumferential) orientation in the SCI bladders [9].
Above 4, some patches opposite the biased orientation remain un-selective throughout training, because very few <img src="http://journals.plos.org/plosone/article/asset?id=info?doi/10.1371/journal.pone.0008778.e129.PNG" class= inline-graphic"/> input patterns will create a leading edge effect to drive bubbles of activity to the opposite edge of the supra-barrels (Fig. 5c).
In addition to the families detected by observation of individual chromosomes, we found patterns of statistically significant biased orientation in ten IS families (table 1).
Elevated temperature in the absence of bias promoted orientation of the BCC Fe phase.
Uncertainty in water contents following this method is dependent on the degree of polarisation of the defects, the absolute water contents and any unintentional bias in orientation due to crystal shape.
Other influences than memory or memory extinction can bias the orientation of bees to a nest location.
At pH 9.0, there was little bias in orientation for the few insertions that occurred in the repeat (Figure 3).
To avoid the bias of orientation errors, only right eyes were included in this study.
Only right eyes were included in this study to avoid the bias of orientation errors.
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