Sentence examples for bias in the sequencing from inspiring English sources
Such deviation, caused largely by GC bias in the sequencing data, placed a practical limit on the sensitivity of the test especially for detecting aneuploidy associated with chromosomes that are GC-poor or GC-rich [3].
Also, the amplification technology can introduce tremendous bias in the sequencing coverage [ 17].
As we will demonstrate in Next-generation sequencing protocol steps prior to sequencing section some of these steps are known to be prone to introducing bias in the sequencing data derived from the sample.
Also, individuals did not cluster predominantly within distinct captures (compare the distribution of symbols within each dataset in Additional file 1: Figure S5), suggesting that the library preparation and hybridization was highly uniform and did not introduce bias in the sequencing output.
Using such a GC-matched background model assures that the overrepresentation of certain motifs is not due to the bias in the sequence nucleotide composition.
Among those 433 samples, 42%, 32%, and 20% of the patients had diseases G, PU, and GC, respectively, which shows that there is a potential for selection bias in the sequence samples.
Two RCTs had a low risk of bias [ 20, 23], but the risk of bias in the sequence generation was high in 2 RCTs [ 12, 15].
Since no amplification is needed it has the benefit that DNA modifications can also be detected and there is no bias in the sequence data.
The amplification step needed to obtain these copies can be a source of bias in the sequence data and information about DNA modifications is lost.
In order to study pattern occurrences in biological sequences, simple frequencies are not relevant in most cases because of pattern overlapping structure as well as composition bias in the sequences.
This potentially introduces a 5' bias in the sequence available for hybridization; however, since the average insert size for all clones is approximately 1.2 kb and most cDNAs were made by oligo dT priming, clones should contain the entire downstream sequence.
