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To reduce any bias in template selection, a group template of images was established directly from the subject dataset.
Nevertheless, if such an intrinsic bias in template recognition exists – whichever its direction may be – it significantly influences the evolution of strand preference.
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Any bias in the template that may have resulted from researcher three's involvement was mitigated by researcher one (MC) undertaking the initial analysis and the team approach to verification of theme generation.
Another important concern in the use of NGS for analysis of environmental samples is the issue of bias in multi-template PCR amplification [ 8].
Polz, M. F. & Cavanaugh, C. M. Bias in template-to-product ratios in multitemplate PCR.
Thus, the bias in favor of templates T and G might be inherent to this particular polymerase active site, and the differences in reaction kinetics are merely exacerbated by mutation of a critical active-site residue.
It cannot be ruled out that the bias resulted from initial inaccuracy in template quantification and/or preparation prior to bisulfite amplicon NGS analysis.
Second, we profiled two intergenic negative control regions which showed negligible amplification in both H3K4me3 and mock ChIP samples, showing that there was no template loading bias in the H3K4me3 samples.
The third crucial parameter is the (intrinsic or possible) bias in the replication of single-stranded templates (i.e. a bias in double strand production from single-stranded templates; k SP ≠ k SM ).
In summary, a bias in the replication of single-stranded templates (i.e. a strand preference in double strand production from single-stranded templates) leads to selection generating anti-correlated strand preference in strand displacement of double strands.
A major source of bias in clinical imaging studies is that template-matching approaches, such as statistical parametric mapping, do not include a head size correction or account for cortical atrophy.
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