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For instance, the Monte Carlo method described in the supplementary material (S1) has the added advantage that it can be used to detect a significant option bias in a single group of animals.
The discrepancy was intriguing and could be due to selection bias in a single hospital or geographic variations.
Furthermore, we acknowledge that assigning trials with high risk of bias in a single domain a status of high risk of bias overall may be arguable.
To evaluate the codon usage bias in a single codon family, we calculated relative synonymous codon usage (RSCU) values of every codon in each sample.
Thus, the bias in a single realization is sufficient to position naive bicoid mRNA, and often also correctly positions oskar mRNA.
After examining biases potentially leading to overestimation they conclude: "A systematic combination of these errors and bias in a single direction (reference [ 3] is cited) may well lead to estimates of cancers attributable to pollutants one order of magnitude larger than the range of reasonably accepted estimates (references [ 1, 6, 7] are cited)".
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It has been observed that, for the case of step-wise increase in gate bias, the IDS transients are slower by many orders of magnitude than if the VGS is directly switched to deep bias (−40V) in a single step.
This bias might in a single study not have any relevance for the interpretation of the results since it is much lower than the standard error of the estimate.
Only 41 hauls from the 2003 2005 data were conducted when wave heights exceeded five feet (1.52 m), however their exclusion reduces the maximum expected bias in any single tow due to wave height by ∼31%.
In this setting, 130-fold amplification produces more than enough RNA for multiple downstream microarray analyses and confers the benefit of reduced bias inherent in a single-round amplification protocol.
One of the limitations of the present study is the selection bias inherent in a single-center retrospective study, although rarity of the disease makes performing prospective studies difficult.
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