As we have shown, the high heterogeneity locus length in the human genome and the uneven distribution of the GO categories in relation to the locus length can bias functional inference.
The method may therefore be more robust to several potential biases which may influence tissue conductivity, thus enabling a low bias functional assessment of muscle fatigue, at different time points, that is, before and after an intervention.
To search for any biased functional distribution, which may indicate functions necessary for a specific phenotype/characteristic, COG functional categories were attributed to each protein in the 58 genomes.
These challenges include the need to detect and quantify various phenomena when screening for allosteric ligands, such as saturability of effect, probe dependence, differential effects on orthosteric ligand affinity vs. efficacy, system-dependent allosteric agonism, stimulus-bias (functional selectivity), and the potential existence of bitopic (hybrid orthosteric/allosteric) ligands.
Also, besides the brain the light travels through all extracerebral head tissues, whose vasomotion biases functional signals.
For example, 454 pyrosequencing technology produces between 11 35% artificial replicates, both identical reads (duplicates) and reads that begin at the same position but vary in length or contain sequencing discrepancies, which lead to biased functional annotations [ 88].
This is particularly relevant since more than half of the patients presented with upper limb dysfunction at disease onset in right and left limb-onset subgroups (16/20, 12/16, resp .. Therefore, we thought it was not adequate to speculate that the ALSFRS in patients with left limb was truly higher because of biased functional disability assessments in our right-hand dominant patients' cohorts.
Furthermore, the transcripts affected by GR QTL did not show any bias in functional annotation, suggesting that polymorphic GR is not limited to any particular annotated class of genes.
We might thus introduce a systematic bias in functional categories associated to subsets of contigs.
However, to account for possible gene intensity bias, further functional enrichment analysis was conducted after respective normalization.
This indicates that this trend, in general, does not alter with the compositional bias or functional characteristics of the genes.
