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The average primary energy use for solders is around 200 MJ per kg, with the high-bismuth solder (58% Bi) only 20% of that value, and three low-bismuth solders (2% to 5% Bi) running very close to the average.
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BI ran for 2*106 generations and trees sampled every 500 generations (burnin = 20 trees).
Regardless of partitioning strategy employed, all Bayesian inference (BI) runs yielded highly reproducible phylogenetic inferences (Additional file 4: Figure S2).
As with all BI runs, nucleotide regions were assigned nucleotide substitution models based on AIC, estimated in jModelTest.
For loci aroE and tpi, the SYM [25] plus Gamma substitution model was used in BI runs.
Two independent BI runs were carried out using random starting trees with one cold chain and three heated chains.
For each BI run, gaps in alignments were treated as missing data.
Within MrBayes BI runs, log-likelihood (lnL) estimates rapidly reached stationarity and convergence.
Each BI run consisted of 4 million generations with every 100 steps being sampled.
An additional BI run was performed for each of the analyses, to confirm the adequate mixing of the Markov chains.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com