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Excluding SE-IPACs and A-IPACs, there are 6053 IPACs located at 700 nt or more beyond the upstream stop codon.
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This means that the SD sequence of this gene is overlapping the upstream stop codon (TGA).
The sequence of the original BamHI/XbaI cloning site and the upstream opal stop codon was prone to mutations in the stop codon to TTA.
This is a clear adaptation of the upstream gene stop codon usage, which becomes part of the SD motif and maintains its function as stop codon.
The selected ORF contained at most two upstream stop signals, and one upstream ORF < 30 codons in length.
Ribosomes not only extend beyond the annotated stop codon of this transcript but also skip subsequent in-frame stop codons present on the predicted 644 nt 3′ UTR.
The URA2 open reading frame is separated by 1231 nt from the stop codon of the upstream gene TRK1.
No further similarity between the upstream sequences was detected beyond this point.
Moreover, a stop codon TAG 12 bp in the upstream sequence makes the start codon of cemA in date palm rather ambiguous.
Translational readthrough gives rise to low abundance proteins with C-terminal extensions beyond the stop codon.
A noise beyond the wall stops them.
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