Sentence examples for beyond the primer from inspiring English sources

In summary, footprinting data on the ternary complexes are consistent with close contact between RT and the first two or three nucleotides of the template extending beyond the primer terminus.

This is consistent with the observation that the excised base from the unlabeled strand (dATP) is complementary to the next base beyond the primer, for this primer/template combination.

Sequences were subsequently filtered to reveal those reads that contained a complete primer including each unique barcode, followed by two bases of transposon (Tn) beyond the primer.

The sequencing primers were positioned such that the first two bases beyond the primers used for PCR would be the last two bases of the transposon.

We used a 529R-containing custom sequencing primer to initiate sequencing beyond the conserved primer sequence region and generate 101 base-pair single-end reads of the V3 hypervariable region using the Illumina GAIIx platform.

An artificial barrier created by binding streptavidin (SA) to a biotin residue in the template caused RT primer extension to stall two nucleotides upstream from the biotin residue indicating that RT protrudes at least two nucleotides beyond the end of the primer.

Pol ι incorporated dC opposite γ-OH-PdG 2 with nearly the same efficiency as opposite a nonadducted template dG; however, further extension of the primer beyond the lesion was significantly inhibited (95).

To evaluate the utility of these primers beyond the target species, the primer pairs were also tested on two individuals from a diverse sampling of Castilleja species representing the morphological and geographic diversity of the genus, with special attention given to the North American species from the rapidly radiating perennial clade of Castilleja (Tank and Olmstead, 2008).

In contrast to the replicative pols, both the yeast and the human variants of pol η can replicate past γ-OH-PdG 2 by incorporating the correct nucleotide, dC, opposite the lesion and extending the primer beyond a dC paired opposite this adduct (71).

A custom sequencing primer (5′-GATCTACACTCTTTC ACCGCGGC[GT]GCTGGC-3′), which contained 529R (italicized), was used to initiate sequencing beyond the 529R primer region, effectively increasing sequencing read length of the region of interest.

Despite this strategy, conservation in 16S sequence beyond the 529R primer region required low cluster density on flow cells for successful sequencing.