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Knowledge about conformational preferences and structural regularities, going beyond the primary sequence
Extra complexity, even for single-subunit proteins, also comes with incorporation of additional structural features beyond the primary sequence, like disulphide linkages, metal ions, cofactors and post-translational modifications, and the use of non-standard amino acids (outwith the main 20).
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B.B. contributed to the primary sequence data analysis.
The primary sequence of the SMUG1 proteins is very different from the UNG-proteins.
The primary sequence numbers of H. sapiens cGAS are labelled at the top of the line.
Shankar Balasubramanian: The primary sequence of DNA can now be rapidly determined for human genomes, enabling DNA sequencing to occur at a larger scale.
For the folding of multidomain proteins, the subdomains act as the primary sequence and their intramolecular interactions drive supertertiary folding.
Note that the amino acid cysteine is absent in the primary sequence of both MBP and GB1.
Beyond their primary sequence, the two most striking conserved features of the AH domain are its high negative charge density and the distribution and orientation of its hydrophobic residues.
It just means the community needs to move beyond primary sequence analysis.
In other words, the identification of true orthology relationships between gene products now should progress beyond primary sequence and "splicing orthology", consisting in ancestrally shared exon-intron structures, is required to define orthologous isoforms at transcript level.
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