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Three-dimensional structured illumination microscopy provides resolution beyond the optical diffraction limit and permits analysis of fluorescently labelled whole cells.
The utility of these probes is underscored in single-molecule and super-resolution imaging experiments, which reveal Na(V) distributions well beyond the optical diffraction limit in subcellular features such as neuritic spines and filopodia.
Metallic nanostructures with localized surface plasmon resonance (LSPR) provide a method for manipulating light at the nanoscale beyond the optical diffraction limit [1, 2].
To characterize the morphologies of Htt exon 1 aggregate species over time and study the fibrillation pathway with spatial resolution beyond the optical diffraction limit of ∼200 nm, we used direct stochastic optical reconstruction microscopy (dSTORM).
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Label-free imaging of living cells below the optical diffraction limit poses great challenges for optical microscopy.
Kumar, K. et al. Printing colour at the optical diffraction limit.
WFMP-FLIM is capable to achieve the optical diffraction limit.
The natural limit is the optical diffraction limit determined by the wavelength and the numerical aperture of imaging system.
The nanoholes appear larger in the optical image than their actual size due to the optical diffraction limit, as shown by comparison with the SEM image.
UV light will also reduce distortions of the laser beams by optical diffraction.
Integrating optical excitation and acoustic detection, PAM has broken through both the optical diffusion and optical diffraction limits.
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CEO of Professional Science Editing for Scientists @ prosciediting.com