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To account for seasonality in malaria and the possibility that the use and perception of rapid diagnostic tests may change over time, recruitment continued for a full malaria season and beyond the minimum sample size.
Our study also suffered from high crossover and contamination, where many patients either visited sites other than the study sites or they visited both the intervention and control sites, reducing the uncontaminated sample beyond the minimum sample size needed to detect the predicted change in outcome variables.
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The last sample size with at least 1 significant result was considered the minimum sample size.
The aim of the second analysis was to discover the minimum sample size.
The minimum sample size = 382.
Therefore, we estimated the minimum sample size at 235 participants.
The minimum sample size required was 385 children.
Thus, the minimum sample size was 148.
Given the minimum sample dilution of 1 25, the minimum quantifiable log titer value was 1.4.
With these premises, the minimum sample size was 139.
The minimum sample size of 645 children was obtained [ 14].
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