Sentence examples for beyond the diffraction barrier from inspiring English sources

Nonetheless, the proposed technologies of super-resolution acoustical focusing beyond the diffraction barrier require complex tools such as artificially engineered metamaterials, and other hardware equipment that may not be easily synthesized or manufactured.

Additionally, photoswitchable or photoconvertible proteins can be employed in novel microscopy concepts that allow imaging of subcellular structures at a resolution beyond the diffraction barrier of optical microscopy [22] [24].

Most recently, a collection of photoswitchable/photoconvertible FPs including KFP, Dronpa, Kaede, Dendra and EosFP and their biotechnologically engineered variants have enabled imaging of protein localization with a resolution beyond the diffraction barrier of optical microscopy [22], [24], [25], [75], [76].

However, for its most popular imaging mode, fluorescence microscopy, the diffraction barrier is crumbling.

According to Professor Stefan Hell of the Max Planck Institute for Biophysical Chemistry, who was the first to break the diffraction barrier in 2000, all superresolution methods rely on the same fundamental principle.

This illustrates the diffraction barrier for conventional optical imaging.

Several approaches have been developed to break the diffraction barrier to achieve super-resolution.

In STED (Stimulated Emission Depletion) microscopy [ 36, 37], two pulsed lasers are used in tandem to break the diffraction barrier.

STED microscopy separates features that are closer than the diffraction barrier by forcing them to fluoresce sequentially.

We have witnessed light microscopy breaking the diffraction barrier, and in the future the spatial resolution will only improve, with the development of super-resolution light microscopes.

To increase spatial resolution, these optical techniques and SPM can be combined, which then permits measurements to be made below the diffraction barrier (within the near-field zone).