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2.5 ml of each alcoholic beverage sample was measured into a measuring cylinder and topped up with de-ionized water to the mark.
The solution was mixed well before use. 1 mL of a well-shaken beverage sample was transferred into a 5 mL volumetric flask.
The optimum number of repetitions of the extraction procedure was determined by extracting 1 mL of the alcoholic beverage sample ten times successively with 1,1,2-trichlorotrifluoroethane.
Three extraction solvents, cyclohexane, n-hexane and 1,1,2-trichlorotrifluoroethane, were compared by extracting 1 mL of an authentic alcoholic beverage sample with different volumes of these solvents.
The ratio of extraction solvent volume/sample volume was chosen by extracting 0.1 mL of the same alcoholic beverage sample with different volumes (0.1 5 mL) of 1,1,2-trichlorotrifluoroethane.
The precision expressed as relative standard deviation (RSD) of the optimized method for DEP never exceeded 9.0% (intraday) and 8.4% (interday) for the authentic alcoholic beverage sample and 8.2% (intraday) and 9.7% (interday) for the spiked sample.
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Blood and beverage samples were analyzed for ethanol concentrations.
The beverage samples were spiked to assess the matrix effect.
The proposed method was applied successfully for the determination of furan in beverage samples.
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