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Specifically, cell number increased equally between vector control and NS5A-expressing cell lines after five days in culture, and >98% of cells were viable by MTT and trypan blue exclusion analyses for all cell lines (data not shown).
Statistically significant differences between vector control and complemented strains were determined using Student's t test.
Furthermore, we noticed a redistribution of caveolin-1 between vector control and PMP22 expressing cells.
The fold-change between vector control and pre-microRNA expression vector was calculated with the 2-ΔΔCt method [ 27, 28].
The unexpected link between vector control and prevalence of drug-resistant malaria parasites in some areas 174– 177 but not others 178 also seems consistent with vector selection.
In addition, there were little to no differences in the cell cycle profile between vector control and wild-type BRCA1 infected cells.
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Two class unpaired Significance Analysis of Microarrays (SAM;[22] with a 0% false discovery rate (q-value) was used to determine differential gene expression between empty vector control and GIPC1 KD MDA-MB231 cells.
It is interesting to note the switch in signaling dependence between the vector control and CD8-IGF1R lines.
In vHMEC, no increases in the frequency of centrosome abnormalities were detected between the vHMEC vector control and vHMEC ras-expressing cell populations (data not shown).
Under the un-induced conditions (26°C), there were no significant differences in AeSCP-2 mRNA levels between wild type (no DNA vector), hsp70 DNA vector control, and hsp70-AeSCP-2-siRNA vector (Fig. 3A, wild type and empty vector).
Purified IgG from vector control and OVA immunized mice were used as negative controls for the viral-vector and protein-in-adjuvant studies respectively.
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