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AUC provides the robust measure for response when single drug is compared between various cell lines at the same concentration range.
The degradation rate of EGFR between various cell lines did not correlate with reduced ErbB2 levels.
Recent studies have revealed that the composition of NPC components varies between various cell lines that have a different state of cell differentiation and also changes during cell differentiation.
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To eliminate possible effects by the Dox treatment or clonal differences between the various cell lines, we only considered those genes which were regulated upon the CNOT2 downregulation (pIND CNOT2 + Dox) compared with both controls pIND CNOT2 -Dox (to eliminate clonal differences) and pIND-Luci + Dox (to eliminate the effects of Dox treatment).
In addition, there were differences in cytokine release and costimulatory receptor expression and function between the various cell lines.
Over-expression of hDsg3.myc did not cause cell death while western blotting for several apoptosis-associated proteins showed no differences between the various cell lines (data not shown).
More plain was the heterogeneity in pathway composition between the various cell lines.
There was no appreciable change in endogenous Pfn1 expression between the various cell lines.
In contrast with the singlet responses, there were marked differences between the various cell lines in the responses to trains of glutamate puffs (Figs 4 and 5).
However, there is no common signal substance upregulated in all investigated cancer cell lines, which leads to the conclusion that the cytokine or chemokine responsible for the increase in the migratory activity either differs between the various cell lines, or is not among the herein investigated signal substances.
In the future, we plan to use the developed AFM platform to investigate the forces between nanoparticles and various cell lines under conditions where certain nanoparticle internalization mechanisms have been blocked.
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