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Significant differences to the rosette EXPni value (FDR <0.05) allowed identifying synergistic interactions (black line) and negative interactions (dashed line) between two transgenic lines.
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Hence, in addition to the difference in H3K9me3 marking, the pattern and level of DNA methylation along repressed hCD2 transgenes also seems to differ between the two transgenic lines.
The changes in the relative mRFP intensities in the germ cells were similar between the two transgenic lines.
This study revealed considerable differences between the two transgenic lines and the wild type (Table 1).
However, a clear difference between the two transgenic lines was also observed.
However, differences could be identified in the diencephalic expression domains (prethalamus and hypothalamus) between the two transgenic lines.
The log-rank test was used to compare significant differences in death rates between the two transgenic lines.
The other interesting transcripts were those that showed differences in abundance between the two transgenic lines expressing either only glycolate dehydrogenase (DEF2) of the full bypass (Full) lines.
For both the CD2 1.3B and CD2 1.3A14 transgenic lines, derepression of the hCD2 transgene was observed with prolonged T cell activation, but the extent of hCD2 derepression differed greatly between the two transgenic lines.
Thus, a direct comparison of these lines by DIC microscopy would effectively be an uncontrolled experiment since there is no satisfactory way to control for exposure time between the two transgenic lines with differing basal expression.
A closer look at the list of 47 genes and their pattern of change revealed that only four genes have the same pattern of change, one up-regulated (At5g47770, FPS1) and three down-regulated (At4g29020, At3g30720, At3g50360) in both COX + and COX++ lines while the other genes showed a reverse pattern between the two transgenic lines.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com