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A smaller horizontal displacement over a given similar duration between two tests is an indication of lesser rough surface.
It is more likely to test an animal in the same (e.g. reproductive) state within a short interobservation interval and the opportunity for developmental change is high when the time between two tests is long [ 28].
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The agreement of dichotomised test results between two tests was assessed using the kappa statistic.
Percent discordance between two tests was calculated as the fraction of total paired observations for which the two methods were discordant among all paired observations.
Agreement between two tests was assessed by Cohen's kappa statistic, with values indicating poor (0.00-0.20), fair (0.21 to 0.40), moderate (0.41-0.60), good (0.61-0.80) and excellent agreement (0.81-1.00).
Third, in 65 among 203 subjects who took both tests, QFT-G was conducted later than TST and in 28 among those 65 subjects, the interval between two tests was over 4 days.
Test C tended to have higher calprotectin values with a lower specificity compared to tests A and B. The concordance between two tests was 0.835 for tests A and B, 0.782 for tests A and C and 0.765 for tests B and C. All three tests are very sensitive for detecting mucosal inflammation, but major differences exist between specificity and absolute values.
A comparison of the time performance between the two tests is summarized in Table 2.
The test-retest reliability was shown to vary between 0.55 and 0.82 when the interval between the two tests was five to eight weeks [ 34].
Discrepant test results between the two tests were confirmed by a μ-capture reference EIA.
In addition, the correlation between the two tests was almost linear.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com