A total of 3,649 genes were found to be significantly differentially expressed in the pair-wise comparisons between any two stages, with 1,800 DEGs (1,154 down-regulated and 646 up-regulated) between the green and yellow libraries (Additional file 6).
To observe bone growth between two stages, we labeled larvae with successive pulses of, first, 50 µg/ml Alizarin Red S, and after a period of wash out, a second pulse of 50 µg/ml Calcein (*high purity*, Molecular Probes catalogue # C481, in E2 embryo medium buffered with 1 mM Sodium Phosphate to pH 8.0).
In between two stages, a mixed transition regime is maintained with a duration Ttr independent of the stage number and of the subsystem.
Note that our multi-layer approach is very different from the two-stage MS/MS search approach with logical dependencies between two stages.
A distance between two stages was 30 cm.
Neutrino's hero is William Willis, who did it in two stages, with eight months in between them.
Root length and diameter were lower at V-stage than the other two stages, with no significant (p < 0.05) difference between B- and S-stage.
We associate the rise in hardness during two stages with the strong strain gradient effects, the first stage is related with the small scale effects and the second stage with the strain gradient between the indenter and the hard substrate.
The rhythm of the festival had been set and for three nights throngs of dancers partied until dawn, racing between three stages under a sky peppered with stars and mirrored by the bobbing lights of fishing boats on the lake.
The simulation differentiates between five stages.
