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In offshore projects, the temporal distance is considered: small when there is a time distance between sites of four hours or less; large when there is a time distance between two sites of more than four hours.
In this analysis we used a rapid and effective method to estimate coevolutionary connection between two sites of a protein family [29].
The highest number of shared taxa between two sites of different regions was found between east and west Atlantic cold seep sites of the Florida escarpment and Regab pockmark, with 7 shared species identified so far (Table 2), and a high level of similarity in the families of polychaetes at the two sites (Table S1).
Interfacility transports between two sites of the same institution were included if the means of transportation involved air or ground ambulance.
Differences in darkness between two sites of the same locality were tested for significance with Kruskall Wallis one-way analysis of variance, and the overall significance was tested with Fisher's combined probability test.
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Note that this calculation considers all possible dispersal routes between two sites for a series of 30 dispersal events.
To account for this, we incorporated the dimension software process distance, which enables the classification of the distance between two sites in terms of the software processes used.
Average walking_dist (Table 4) again differed significantly between seasons (p < 0.001) and between the two sites of the study area (p < 0.001).
Furthermore, we could also identify potential allosteric movements caused by genistein binding to the wild type TTR that explains, at least in part, the frequently observed negatively cooperative process between the two sites of TTRwt when binding ligands.
No difference was observed between the two sites of stimulation and thus data were pooled together.
The mean distance between the two sites of interest was however 28 mm with a small SD of 4.9 mm, which is large enough to assume that stimulating one area does not directly affect the other [41].
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