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An additional assumption that may need to be relaxed is the independence of the edge failures, which may not hold in cases where the loss of an interaction between two proteins causes a significant destabilization of the larger complex they belong to.
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Next, superimposing protein structures by their main/large domains allowed us to examine conformational differences between two proteins caused by the swapping phenomena on a solid basis.
Kain and colleagues hypothesise that molecular mimicry between the two proteins causes antibodies to LAMP-2 in susceptible individuals after infection with fimbriated bacteria, and this in turn causes AAV (as illustrated in Figure 1).
That the two Nv-NF-κB proteins show a difference in migration on SDS-polyacrylamide gels (Figure 5A) suggests that amino acid differences between the two proteins cause them to have local structural or charge differences as well.
The resulting instability of Tim10 in the Tim9 mutant strains may then be due to poor interaction between these two proteins, causing Tim10 to exist primarily in monomeric form and thus be vulnerable to digestion by Yme1.
This finding establishes a link between two proteins that can cause amyotrophic lateral sclerosis when mutated, VAPB/ALS8 and p97/ALS14.
These results suggest that the salt type used can cause a dramatic difference in the level of binding between two proteins.
Conversely, an edge between two proteins can also be placed if the two proteins share a common trait, such as being targeted by the same drug or causing the same disease.
Its sugar-binding protein Lmo2125 has 63 percent identity/77 percent similarity to the B. subtilis protein, the observed differences between the two proteins seem to cause a sufficient affinity for maltose in the case of L. monocytogenes.
Most drug trials to date have targeted amyloid and tau, two proteins that cause plaques and tangles in the brain.
Because both proteins were expressed properly, the observed differences between the two proteins were most likely not caused by defective protein expression.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com