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Although it was recently suggested that AP2G transcription may alter in response to different environments33, the distributions of relative transcript levels were not significantly different between two populations with respectively low and high endemicity here.
With a standard deviation of 3.0 h, the minimum number of rhythmic nematodes to detect a significant difference between two populations (with 2 hours difference in average) would be 36 [236.
Only those spots with over a 1.5-fold change in volume between two populations, with p values less than 0.05 (p<0.05) in Student's t-test for the variance of these ratios for each protein pair across three independent gels, were defined as significantly different, as described previously [34], [35].
The second model, parapatric or sympatric speciation, involves cessation of gene flow between two populations with overlapping geographical range [ 4- 6].
(D ) In competitive communities, the ratio between two equally-fit populations remained steady (grey), whereas the ratio between two populations with unequal fitness (G 20% fitter than R ) changed monotonically (black) in favor of the fitter population.
This suggested that assortative mating could be based on non-visual cues, so we further examined the sensory basis of assortative mating between two populations with different male colour.
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For this calculation, we used the formula to test the difference of proportions between two populations [ 50] with a = 0.05 (one side) and the power of 80%.
Statistical differences between two populations were analysed with either an unpaired t-test for data analysed for Gaussian distribution, or a two-tailed Mann–Whitney test for unpaired observations in all other cases.
At the same manner, the chondrogenic potential measured after 21 days of culture in chondro-inductive condition, was similar between the two populations with significantly higher amount of glycosaminoglycans in comparison with the respective controls (2.9 fold in TCs- and 2.6 fold in TCs+, p < 0.05, Fig. 2c, d).
The Pb isotopic ratios also differed between these two populations, with ratios in the northern population being closer to that of Pb ammunition signatures [3], and similar results have been obtained when comparing other Canadian populations [4].
The absence of decrease in the number of β-galactosidase hepatocytes in these animals in which a proliferation of labeled oval cells occurred, strongly suggested a lineage relationship between the two populations with labeled oval cells giving rise to labeled hepatocytes.
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