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This analysis assumes that two pathways are likely crosstalk if significantly more proteins or protein interactions are detected between two pathways than expected by chance [27].
Accumulating evidences suggest that a cross-talk between two pathways exists in cells as the active caspase-8 cleaves a BH3-domain-only subfamily protein, Bid.
The total energy of the system was defined in terms of the distance of each pathway from the center, overlapped areas between two pathways, and between a pathway and the periphery of the figure (see Text S1).
Several studies indicated that signal amplitude and duration are temporally modulated by cross-talk between two pathways (e.g. Raf inhibition by Akt) [14], [15] and inhibitory feedback from ERK to Grb2-SOS complex formation [16], [17] and Raf [18] [20].
Among 18 SLGIs between two pathways, 11 are novel predictions.
The presence of a common element between two pathways can serve to couple them.
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On the other hand, gene expression profiles may indicate a non-sequential relationship, such as a feedback circuit between two pathway members.
When cut-off value of 0.5 was used to distinguish if the two pathways interact with each other, 41 out of 45 pathway pairs are consistent between two pathway databases.
There is also an exchange of information between the two pathways via many connections between different areas.
Furthermore, no direct comparison has been made between formation times, nor has the branching ratio between the two pathways been examined under strong-field conditions.
Intriguingly, ABA signaling inhibits the BR signaling pathway after BR perception, and crosstalk between the two pathways upstream of the BRASSINOSTEROID-INSENSITIVE 2 (BIN2) kinase has been reported27,28.
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