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A PCR-based marker system was used to detect polymorphism between two parental isolates.
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Phylogenetic reconstruction based on these sites confirmed separate clades corresponding to the two parental isolates (supplementary fig. S1, Supplementary Material online), with an estimated 268 SNPs between the two clades and one or less core genome SNPs per strain within the individual progeny clades (supplementary table S3, Supplementary Material online).
Schröder, M. S. et al. Multiple origins of the pathogenic yeast Candida orthopsilosis by separate hybridizations between two parental species.
Protein engineering with homologous recombination develops chimeric proteins by swapping fragments between two parental proteins.
This study is original since hybrid crows were intermediate between the three types of crows produced by the two parental subspecies (since the European quail produces two different crows) and not between two parental signals as usually observed.
The markers between two parental genetic maps were linked using custom perl scripts, and Circos was used to visualize the collinearity of two parental maps.
In these studies, a putative SFP is identified based on signal differences between two parental lines.
gDMRs are CpG rich, and differential DNA methylation is observed between two parental alleles.
For example, Helianthus anomalus is a wild sunflower species derived via hybridization between two parental species, and its genome is characterised by parental species blocks.
In this study, we sequenced four T. parva isolates using the Roche 454 GS FLX Titanium chemistry (Table 1), which included two parental parasite isolates, Marikebuni and Uganda, and two recombinant clones derived from experimental co-infection experiments of cattle.
PC1, which explained 70.9% variance, represented a large part of the difference between the two parental genomes.
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