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These findings, however, were not consistent between two independent experiments.
In all three studied markers, TD, MSAP and TMD, changes in the calli and/or regenerated plants relative to the donor plants for a given genotype, which were reproducible between two independent experiments, were scored.
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Error bars indicate standard deviation between three independent experiments measured in triplicates.
The error is the standard deviation between five independent experiments and includes the statistical plating error.
Assignments were made such that no sample had the same tag identity between three independent experiments.
IC50 values were calculated for each cell line from survival curves using data generated from between two and seven independent experiments.
In protein recruitment experiments, signal intensity was quantified along the laser path, using Slidebook 6 software, in a minimum of 10 cells and error bars represent the SD between three independent experiments.
Excellent agreement was observed between these two independent experiments.
The mesocotyl and coleoptile lengths were not significantly different between the two independent experiments (r = 0.79 for mesocotyl, r = 0.66 for coleoptile; P < 0.001).
The correlation between the two independent experiments in C. reinhardtii was relatively high (i.e., correlation coefficient = 0.76), indicating a good quality of the two datasets.
However, the low concordance between the two independent experiments highlights the complexity of the chromatin landscape and the multitude of regulatory factors involved in determining transcription and phenotype.
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