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Expression analysis using low temperature-treated whole-genome microarray data revealed variable transcript abundance of 1R/2R/3R/4R/5R-MYB genes in 11 clusters between two inbred lines of Chinese cabbage, Chiifu and Kenshin, which differ in cold tolerance.
Here, the hybridization data from microarrays not only measure differential gene expression but also can yield information on sequence variation between two inbred lines.
AILs are generated by randomly and sequentially inter-crossing a population initially originating from a cross between two inbred lines (Darvasi and Soller 1995); however, in our case multiple parents are used.
Non-founders (n1 = 301) were bred via repeated self-pollination of a hybrid between two inbred lines.
A RILs population consisted of 201 F10 recombination inbred lines, derived from a cross between two inbred lines, Huangzao4 and CML288, using a single-seed descent method under short-day conditions (Sanya, China, 18°45'N, 109°30'E).
A genetic map of Brachypodium distachyon has been created from 183 F2 individuals derived from a cross between two inbred lines Bd3-1 and Bd21 [15].The map consists of 139 marker loci distributed across 20 linkage groups with a combined length of 1386 cM.
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Rebaï and Goffinet adapted the linear regression method to the case of a diallel cross between four inbred lines [7], and afterwards presented a general linear model (GLM) for QTL mapping by combining different populations derived from diallel designs [8].
Then, we used the eigengenes to represent the modules to calculate the Pearson correlations for each pair of modules between the two inbred lines.
These results suggested that the difference in EC induction capability between the two inbred lines was very complex and involved various metabolic pathways.
No significant difference was observed in uninfected mouse brains between the two inbred lines (P = 0.12).
Our results indicate that besides the previously-reported PAVs, many genes show different expression patterns between these two inbred lines.
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