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CoGe's tool SynMap [ 44] identifies orthologous genes between two genomes using sequence homology and synteny.
We performed similar sensitivity analyses for other parameters and present those results in Additional File 1. Syntenic analysis was conducted between two genomes using orthologous genes as anchors.
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This difference was used to calculate pair wise distance between the two genomes using two different approaches.
To adjust for this, the UCSC conservation track, which is the best alignment between two genomes, is used instead.
To illustrate the genomic differences, we summed the length of indels found in a mitochondrial genome comparison and defined a dissimilarity indicator to measure the divergence between two mitochondrial genomes using indels and single nucleotide polymorphisms (SNPs) between them.
The list of orthologs between two genomes was identified using reciprocal best hits with more than 80% similarity in protein sequence and less than 20% difference in length, as in Rocha, Touchon, et al. (2006).
The Ks distribution between two genomes can be used to estimate the divergence date between the two species; the mode(s) of the distribution represents either a speciation or duplication event.
The distance between two genomes is often computed using only the common markers, that occur in both genomes.
The KS-test was employed to assess the deviation between the number of counts per bin that occurred between two genomes (one test, one reference), using the maximal deviation (D-statistic) between the cumulative count distributions of the populations being compared (for a visual demonstration of the D-statistic, please see the inset on Figure 4A).
The delta distance (Mrazek 2009) between two signatures is defined as following: (9) The delta distance between two genomes G1 and G2 was calculated using all pairs of nonoverlapping 50 kb segments.
Hierarchical clustering (UPGMA) of the 29 strains was performed using a distance between two genomes based on gene content (binary data for presence or absence of each protein family) measured by one minus the Jaccard coefficient (Jaccard distance).
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