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We then compute the similarity between two genes based on the overlap between their GO profiles.
These scores were computed using a probabilistic approach that assigns a score to each interaction between two genes based on their likelihood of participating in the same biological process (See Additional File 1 Supplementary Methods).
To facilitate the comparison of genomic datasets across species, we converted all datasets into network representation using a probabilistic approach that assigns a score to each edge (interaction) between two genes based on their likelihood of participating in the same biological process [ 24] (see Methods and Additional File 1).
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A past study proposed a method to measure a functional relationship between two gene sets based on protein interaction network [ 35].
As proposed in Ma et al. and Barker et al. we propose a biologically motivated approach to measure the relationship between two different genes based on their temporal expression profiles in RNA-seq.
Briefly, in Part A, the expression similarity between two genes is calculated based on two factors: their steady-state mRNA expression levels, recorded by microarray data, and their potential from microRNA-mediated posttranscriptional regulation, as indicated by their microRNA binding sites.
In general, high first-order similarity suggests the existence of a functional link between two genes, and clustering based on the second-order similarity captures multiple functional links always activated and deactivated under similar conditions.
The GO similarity score between two gene products is based on the number of shared ancestors.
The putative replication origin (oriC) of JL03 chromosome was identified between two genes, gidA (APJL1688) and cof (APJL1689), based on GC skew and the presence of DnaA protein recognition sequences (DnaA-boxes) [12], [13] with typical gamma proteobacterium oriC features as what found in other genera of the Pasteurellaceae family (Figure 1B and Table 1).
A relationship was defined as common when a relationship between two genes that was identified in PA (based upon the DEG list) was also present in one or more other tumor types (GBM or oligodendroglioma).
The genomic distance between two genes is the number of base pairs between their start locations.
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