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Time interval between two cortisol sampling was (median, IQR) 12 (8-30) days.
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For all subsequent analyses, the average of all seven cortisol samples was used unless otherwise specified.
It is noteworthy that only the average of all seven cortisol samples was correlated with rACC volume, while the average of the morning samples was not.
The cortisol response to social stress was measured based on four cortisol samples.
However, the effects of prenatal and postnatal Pb exposure on cortisol reactivity were not significantly different for the three cortisol samples.
The primary measure was CAR measured as the difference between the two salivary cortisol samples.
The primary cortisol measure of interest was the CAR, derived using the difference between the two morning cortisol samples.
Two saliva cortisol samples per pig were collected: one basal sample in the home pens prior to clinical inspection (between 06:00 and 07:00 h), before the normal feeding time of the pigs, and a post-transport saliva sample after arrival and mixing at the slaughterhouse (between 08 00 and 09 00).
There were also no significant differences between the three groups in mortality, interval between the two cortisol tests, or disease severity (SOFA and APACHE II scores).
Before adjustment for multiple comparisons, there was also a significant interaction between SUD and cortisol sampling year predicting logAUC (p = 0.03) (Table 3).
Patients were excluded if there was clinical improvement (defined as a decrease in Sequential Organ Failure Assessment [SOFA] between the two cortisol level tests) and if they underwent steroid treatment before the second cortisol level determination.
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