The degree of similarity, defined as the percentage of points that are similar between two contours, was used as a quantitative measurement of the performance of the segmentation algorithm.
Then, the Euclidean distance between the two normalized contours is computed.
The normalized distance d m,l (t' + 1, t') between y m,t' + 1 and y l,t' + 1 can be calculated from the intersection area between two contours.
HD measures the maximum and minimum distance between two contour sets, and it can be used as metric of similarity between two contours superimposed together.
This current function is such that between the two contours there is a current flow of, parallel to the contours, clockwise round a minimum.
Classically, interpolation between contours is performed by triangulation techniques or by creating intermediate contours with dynamic elastic interpolation.
Two contour fragments are relatable when they can be connected with a smooth contour without inflection points [71].
These three types of contours are visualized in Fig. 2.
Fluorescence and corresponding phase contrast images between two transferring cells (green and orange contours) are shown.
