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The upper limit of the separation guard is the duration of the preamble, namely 6.375 s, because this is minimum time between two consecutive trials without resting time.
A fixation cross was presented between two consecutive trials for 20 seconds and served as our baseline.
As is displayed in Fig. 1d, the ITD is the minimum time that will elapse between two consecutive trials set by the researcher.
Thus the mice could transition immediately from sample to trial runs, but experienced a forced 10 min ITI between two consecutive trials.
This resulted in eight different trial types based on the relationship between two consecutive trials in terms of task, response, and S-R compatibility.
More specifically, greater values of fd1 that signify larger stimulus frequency differences between two consecutive trials create increased mismatch conditions for the subject to correctly identify the present stimulus frequency.
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The animals were tested until they showed no further improvement in the performance, which means the variation between three consecutive trials was less than 30%.
Time was added to the interval between any two consecutive trials according to the stability of the eyelid position signal: if the eyelid position signal (the 'baseline' signal) strayed outside an experimenter-determined range during 1 s prior to the planned delivery of the CS, an additional 1 s was added to the intertrial interval until this criterion was met, after which the trial was initiated.
The stability of direction-tuned cells was evaluated by correlation of either the spikes in each half of a trial (within-trial stability) or the spikes of two consecutive trials (between-trial stability).
Across-trial stability was defined as the correlation (Pearson's r) between spatially corresponding bins from two consecutive trials.
The inter-trial interval, that is, the time between the onsets of the words of two consecutive trials, was 6 seconds.
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between two consecutive updates
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