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Finally, it could be argued that there is no direct proof that the serotype changes reported in the studies we selected are indeed due to capsular switch, as many events can go unobserved between two consecutive samplings.
The antibodies specific to PCV2 were measured in individual serum samples diluted in twofold dilutions from 1 10 to 1 20,480 (Log 1.0 to Log 4.3), The results are presented as Log 10 levels of the antibody titres and seroconversion between two consecutive samplings was defined as an increase with at least two titre steps, corresponding to an increase with at least Log 0.6.
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It stabilizes the system when the maximal time between two consecutive sampling instants is sufficiently small.
In addition, when a contact-infected pig showed HEV RNA excretion at two consecutive samplings, a control sample was taken from the next-generation contact pig.
Moreover, when two isolates of the same serotype were detected in two consecutive samplings, PFGE analysis frequently showed that the serotypes were genetically different.
We defined prolonged hyperglycaemia as BG values higher than 2 g/l more than 50% of follow-up time (with linear interpolation between two consecutive blood samplings).
Repeat testing has been advised every 8 12 h for at least three consecutive samplings [ 13, 14, 67].
In case of persistent suspicion and negative microscopy results, repeat testing every 8 12 h for at least three consecutive samplings is recommended.
When the C1-pig excreted HEV in faeces at three consecutive samplings it was moved to the compartment containing the second-generation contact pig (C2-pig).
When the C2-pig excreted HEV in faeces at three consecutive samplings it was moved to the compartment containing the third-generation contact pig (C3-pig).
T The sampling interval between two consecutive scans.
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