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However, it can be difficult to interpret a difference in absolute rate between two cell populations.
To analyze cell-cell interaction between two cell populations, each population must be separately collected after cellular cross talk.
We have described the expected patterning between two cell populations if the fitness effects of interactions are the major driving force.
Logistic regression linear classifiers were used to infer the best predictor genes in the separation between two cell populations (Teles et al., 2013).
The statistical significance of the difference between two cell populations was determined using the two-tailed Student's t-test (Microsoft Excel).
The statistical significance of the differences between two cell populations was determined using the two-tailed Student's t-test (Microsoft Excel) and a P value < 0.01 was considered statistical significant.
Similar(50)
Neither could we detect any evidence for variability in the abundance of NuRD component proteins between these two cell populations or between serum/LIF conditions and 2i/LIF conditions.
The magnitude of divergence in transcriptional profiles between normal chondrocytes and the cells that populate repair tissue reveal substantial functional differences between these two cell populations.
Using this optimized experimental protocol in combination with our microarray analysis methodology we find 49 differentially expressed genes between the motor neurons and the interneurons that reflect the functional differences between these two cell populations in generating and transmitting the motor output in the rodent spinal cord.
Moreover, using the engineered co-culture platform, the paracrine activity was found to be dependent on the distance between the two cell populations and be efficient at a narrow gap width (500 μm).
Osteopontin was observed mostly at the interface between the two cell populations.
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